Sequencing Purified clone/ Plasmid DNA

Single sequencing reaction
550-650 bases data

SB/SEQ 01

Sequencing of plasmids (unpurified)

Send  clones  as  stabs/ slants/ culture plates, we purify DNA
We send 96-well plates with LB Agar + antibiotics, for large no. of samples
Good data guaranteed for >95% samples

SB/SEQ 01A (<100 samples)

SB/SEQ 01B (<1,000 samples)

SB/SEQ 01C (>1,000 samples)

Sequencing Purified PCR products

Single pass analysis of 550-650 bases data

SB/SEQ 02

Sequencing PCR products (unpurified fragments)

 Send unpurified PCR samples in PCR vials only
PCR Clean-up/ Gel elution of specific band are performed
Good data guaranteed for >95% samples

SB/SEQ 02A (<100 samples)
SB/SEQ 02B (<1,000 samples)
SB/SEQ 02C (>1,000 samples)

16s rDNA/ 18s rDNA/ ITS Sequencing from Specimen

We isolate gDNA from specimen: bacteria, fungi, plant and animals
We perform PCR using in-house consensus primers and sequence
Sequence data aligned and delivered
Sequencing would fail for mixed cultures received, will be charged in full

SB/SEQ 11A

Genomic DNA Isolation from all Biological Samples

Column based protocol: RNase-free, DNase-free gDNA
MiniPrep, upto 5 µg
MidiPrep, upto 100 µg
MaxiPrep, upto 500 µg

SB/GDI 01
SB/GDI 02
SB/GDI 03

PCR Standardisation

Standardisation for amplification of Microsatellites, variable regions, homologus genes, novel genes, etc

SB/PCS 01

PCR and Sequencing

Perform PCR and Clean-up from Genomic DNA/ Plasmid DNA/ template DNA
Primers and PCR amplification conditions provided by the Scientists
Full-lenght sequencing of amplicon
Data aligned and provided
In case PCR fails (upon repeated attempts), we charge

 SB/SEQ 10

SB/SEQ 10F

Primer-walking (Single-Strand)

Single Strand sequencing, charged per base
Internal primers are synthesized
Final data aligned and delivered

SB/SEQ 04

Primer-walking [(AT/ GC) % >70%] Single strand       

Single strand  

SB/SEQ 04A

Primer-walking (Double-strand)

Double Strand sequencing, charged per base
Internal primers are synthesized
Final data aligned and delivered

SB/SEQ 05

Primer-walking [(AT/ GC) % >70%]

Double strand

SB/SEQ 05A

Sequencing Native bacterial plasmids  

Provide pure Plasmids
Complete Circular map provided
Restriction map provided

SB/SEQ 08

SNP detection and data analysis

Sequencing-based protocol
Bi-directional sequence data to confirm SNPs, charged extra
SNPs marked on peak data Zygosity detected based on reference sequence data provided
Primers and PCR amplification conditions provided by Scientist
Primers, if required, are charged extra
In case PCR fails (upon repeated attempts), we charge 

SB/SEQ 09

SB/SEQ 09F

 HLA Class I Typing

HLA-A/B/C typing by sequencing 
Exon 2 and 3 amplified
Genomic DNA isolation included
Sequence data provided

SB/HLAC 1

HLA Class II Typing

HLA- DRB1/ 3/ 4/ 5/ DBP1/ DQA1/ DQB1 typing by sequencing 
Exon 2 and 3 amplified
Genomic DNA isolation included
Sequence data provided

SB/HLAC 2

SSCP analysis

SB/SSCP 01

PCR, Cloning & Sequencing

500 bp or smaller amplicons, 1- 4 samples
500 bp or smaller amplicons, >4 samples
500 bp to 1.0 kb amplicons, 1- 4 samples
500 bp to 1.0 kb amplicons, >4 samples
1.0 kb to 2.0 kb amplicons, 1- 4 samples
1.0 kb to 2.0 kb amplicons, >4 samples

SB/CSS 01
SB/CSS 02
SB/CSS 03
SB/CSS 04
SB/CSS 05
SB/CSS 06