Genotyping Services Specifications Catalogue No

Development of Microsatellite 

Microsatellite library from for any organism 20, 30, 40 or 50 Markers are developed,  Charges vary
Marker specific primers designed  and PCRs standardized
All marker sequence data, primer-pairs and protocols delivered

SB/SSR 01 (20 Markers)

SB/SSR 02 (30 markers)

SB/SSR 03 (40 markers)

SB/SSR 04 (50 markers)

Genotyping by SSR/ STR analysis

Once the Microsatellite marker data is available (Service Cat. # SSR 01), genotyping can be initiated 
Genomic DNA isolated from individual sample
STR specific PCRs are performedusingflurescent primers sample 
PCR amplicons are sized on a Genetic analyzer

For studying 5 markers in 100 plants, No. of markers is calculated as: 100 x 5 = 500 markers

Synthesis of Fluorescent primers and PCR standardization are charged extra

SB/SSR 05 (<100 markers)

SB/SSR 06 (<1,000 markers)

SB/SSR 07 (>1,000 markers)

Fragment Analysis by GeneMapper/ GeneScan

Fluorescent-tagged PCR products are sized on Capillary Sequencer
Compatible dyes are:
DS-33 (Dye set G5): FAMTM, NEDTM, VIC®, PET® and LIZ® 
DS-30 (Dye set D): FAMTM, HEXTM, NEDTM, and ROXTM
Services include: Performing PCR amplification, multiplexing (upto 4 Markers), Adding LIZ-500, Sample denaturation, Sample Run and Sizing fragments
Sizing data and Chromatogram are provided. 

SB/STR 01 (<100 runs)

SB/STR 02 (>100 and <1,000 runs)

SB/STR 03 (>1,000 runs)

Genotyping  by AFLP

Polymorphism analyses within segregating  populations, varieties, strains, etc.
Complete protocol starting from gDNA isolation to selective PCRs 
Guaranteed  polymorphism; Fluorescent  selective primers are charged extra


AFLP Fragement Analysis

Fluorescent–labeled AFLP profiles analyzed
Compatible dyes are:
DS-33 (Dye set G5): FAMTM, NEDTM, VIC®, PET® and LIZ® 
DS-30 (Dye set D): FAMTM, HEXTM, NEDTM, and ROXTM
Pair-wise sizing data, phylogenetic tree and Chromatogram provided.


Genotyping  by RAPD
(Non- Fluorescent primer)

RAPD analysis using 20-mer primer
Annealing at 55ºC, thus reproducible  profiles 20-30 bands on profile
Polymorphism  generated within closely related samples as well
Primer information, Agarose gel photo, etc. provided
Phylogeny  analyses is difficult


Genotyping  by RAPD
(Fluorescent primer)

RAPD  analysis using fluorescent  labeled 20-mer primer
Annealing at 55ºC thus reproducible profiles
RAPD profile analyzed on Genetic Analyzer
50-75 bands on profile
Polymorphism  generated within closely related samples as well
Phylogeny analyses is assured



Development of ARMS Marker

Distinguishes two similar genotypes
Primer-sets pecific to a genotype developed and validated
RAPD based  polymorphism detected 
Polymorphic bands cloned and sequenced
ARMS primers designed and validated


Authentication of Genotype by Barcoding

Profile developed using fluorescent labeled RAPD primer
Represented genome wide variation with ~1000 loci data


Development of SCAR Markers

Genotyping of closely related samples
Polymorphic RAPD profiles are  developed for  each sample
Sample  specific  markers/ primer-sets are  designed and    Genotypes  are validated


SNP detection and data Analysis

Sequencing-based protocol
Bi-directional sequence data to confirm SNPs, charged extra
SNPs marked on peak data Zygosity detected based on reference sequence data provided
Primers and PCR amplification conditions provided by Scientist
Primers, if required, are charged extra
In case PCR fails 9upon repeated attempts), we charge







MicroArray& Genome Sequencing Services

Microarray service for detection expression level changes, aCGH, ChIP on chip, Methylation profiling and  miRNA profiling

SB/MAS  01

Trnscriptome Sequencing: cDNA library preparation 
cDNA  library  preparation for transcriptome Sequencing using  Illumina solexa platform, Provide  fresh  samples, collected on dry-ice

SB/TLP  01

Genome Sequencing: Basic Sequencing and data analysis:
Raw  data/basic  sequence analysis without reference genome
Functional analysis

SB/SDA  03


Genome Sequencing: Solexa Illumina Protocol
Paired  end  (per  lane)  @ 72 bp  read  length

SB/SS 02

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