Bacterial Identification

Protocol works for pure cultures only
16S rDNA based molecular Identity
~1,400 bp sequence data
Phylogenetic Tree and 10 nearest neighbour info

SB/BIS 01

Bacterial Population analyses : Determination of different types of Bacteria in a consortium

RFLP-based molecular protocol
Minimum no. of bacteria present predicted

SB/BIS 06

Identification of Bacteria in a consortium 

Studies of mixed populations within Eco-samples & slurries
16S rDNA gene-pool cloned and screened
All independent clones are sequenced and ~1,500 bp sequence data provided
Phylogenetic Tree and 10 nearest neighbour info for all bacteria provided
Charges differ based on no. of bacteria present in sample

SB/BIS 02
SB/BIS 03
SB/BIS 04
SB/BIS 05

Fungal Identification Service

ITS region based  molecular identity
Raw and aligned sequence data provided
Phylogenetic Tree and 10 nearest neighbor information provided

SB/FIS 01

Fish Identification Service

COI region sequence-based  molecular identity information provided
~500 bp sequence data, Phylogenetic Tree and 10 nearest neighbour info provided

SB/FIS 02

Plant Identification Service

ITS region or 18S rDNA based Molecular Identity
Phylogenetic tree and 10 nearest neighbour info provided

SB/PIS 02

Development of Recombinant Microbes: 
By introduction  of  foreign genes

A gene or set of genes are cloned and sequence confirmed
The genes / gene-set can be inserted within the target genome(another microbe)
Location-specific insertion on genome is possible
Choice of promoters and terminators available

SB/MUT 03

By Knocking out specific gene(s)

A gene or set of genes are cloned and sequence confirmed
The genes / gene-set can be inserted within the target genome (another microbe)
The deletion would be gene specific/site specific

By random mutation of gene (s) 

A particular gene/genes within the genome can be mutated randomly
First, the gene(s) are cloned, characterised and mutated randomly
Mutated genes are inserted within target genome individually
Stable mutants delivered 

Methylation Analysis by Bisulfite Sequencing

Primer design and DNA extraction
Bisulfitr conversion of unmethylated Cytosines
PCR amplification and gel analysis of PCR products
Subloning of PCR products or direct sequencing (ABI Prism^TM  3500 xl DNA Sequencer)
Sequence comparison and analysis of 5MeCpGs

 SB/BSQ 01