PCR Enzymes and Kits: FastPCRTM

          FastPCRTM Reagents: Perform routine and complex PCRs in flat 40-50 min.
          Get 4 to 5 FastPCRTM runs in the time of one Standard PCR run
          Fast Q-PCR Reagents: Perform all routine and complex Q-PCRs in Fast and Standard formats

Product Specifications Catalogue No Pack Size

FasTaq™ Polymerase

Polymerizes @ 500 bp/ sec
Just 1 sec extension for 500 bp amplicon
Just 2 sec extension for 1 kb amplico
FastPCRTM cycle condition: 500 bp
http://www.chromous.com/sites/default/files/pcr-1.png
FastPCRTM cycle condition: 1 kb
http://www.chromous.com/sites/default/files/pcr2.png
Fidelity similar to Taq Polymerase
The enzyme works in all standard PCR Machines
Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc.
PCR Controls (template and primers) provided

SB/FCN 01

300 Units

 

 

FasTaqHiFi™ Polymerase

Fidelity similar to Pfu Polymerase
Polymerizes @ 500 bp/ sec
Just 1 sec extension for 500 bp amplicon
Just 2 sec extension for 1 kb amplicon
FastPCRTM cycle condition: 500 bp
http://www.chromous.com/sites/default/files/pcr-1.png
FastPCRTM cycle condition: 1 kb
http://www.chromous.com/sites/default/files/pcr2.png
The enzyme works in all standard PCR  Machines
Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc.
PCR Controls (template and primers) provided.

SB/FCN 02

 

300 Units

 

FastLong™ Polymerase

FasTaq and FasTaqHiFi Enzyme mix for better fidelity
High Fidelity: 13x10-6
Polymerizes @ 500 bp per sec.
Just 30 sec extension for 5 kb amplicon
FastPCRTM cycle condition: 5 kb
http://www.chromous.com/sites/default/files/pcr-3.jpg
The enzyme works in all standard PCR Machines
Amplifies upto 5 kb
PCR Controls provided

SB/FCN 03

300 Units

FastPCR™ Master -Mix

FasTaqTM Polymerase –based kit
FastPCRTM Cycle conditions: 40 min for 30 cycle PCR
All PCR components added at 2X concentration, just add template,
primer and water to start FastPCRTM 

SB/FCN 04

5 x 1 ml

         Standard PCR

Product Specifications Catalogue No Pack Size

 

 

 Taq DNA Polymerase

3U/ µl, buffer with MgCl2, PCR Controls provided

SB/PCN 01

300 Units

3U/ µl, buffer with MgCl2, PCR Controls provided

SB/PCN 02

2 x 300 Units 

3U/ µl, buffer with MgCl2, PCR Controls provided

SB/PCN 03

5x 300 Units

1U/ µl, buffer with MgCl2, PCR Controls provided

SB/PCN 07

2 x 300 Units 

1U/ µl, MgCl2 provided separately, PCR Controls provided

SB/PCN 08

2 x 300 Units 

1U/ µl, MgCl2 provided separately, PCR Controls provided

SB/PCN 09

5 x 300 Units 

3U/ µl, MgCl2 provided separately, PCR Controls provided

SB/PCN 04

300 Units

3U/ µl, MgCl2 provided separately, PCR Controls provided

SB/PCN 05

2 x 300 Units 

3U/ µl, MgCl2 provided separately, PCR Controls provided

SB/PCN 06

5x 300 Units

 

GreenTaq™ Polymerase

Green colored Taq for easy viewing and loading
3U/ µl, Buffer contains MgCl2 
PCR positive controls provided with every purchase   

SB/PCN 13
     

300 Units

Green colored Taq for easy viewing and loading
3U/ µl, MgCl2 provided seperatly                     
PCR Controls provided with every purchase

SB/PCN 14

300 Units

Fidelity Polymerase

Highest known Fidelity: 1.5 x 10-6 (1.5 mutations in a million
bases incorporated
Similar to Pfu Polymerase
PCR positive Controls provided with every purchase

SB/PCN 10    

300 Units 

ChromTaq™ Polymerase

Enzyme mix of Taq polymerase and Fidelity polymerase
High Fidelity: 13 x 10-6
Can be used for all regular PCRs
Amplifies up to 5 kb
PCR positive Controls provided with every purchase

SB/PCN 11

300 Units

XTRA™ Polymerase

For 3-20 kb amplification
For usage with AutoExcel PCR Protocol
PCR positive controls provided with every purchase

SB/PCN 12

300 Units

PCR Master- Mix (2 X)

Taq Polymerase-based kit
2X-mix (make up volume with template, primers and water)

SB/PCN 24 

5 x 1 ml

ChromTaq Master-Mix

 

ChromTaq-based high fidelity PCR kit, provided as 2X
2X-mix (make up volume with template, primers and water)

SB/PCN 17

5 x 1 ml

GC-PCR Solution

 

Good for GC-rich repeat amplification (Fragile-X)
Good for amplification of GC-rich genes
(genes from M. tuberculosisgenome)

SB/PCN 18

1 ml

Long-PCR Master-Mix

5-20 kb amplification kit for plasmids/ genomes

SB/PCN 20

5 x 1 ml

GreenTaq Master Mix

GreenTaq™  Polymerase-based kit
2X-mix (make up to volume with template, primer and water)

SB/PCN 19

5 x 1 ml

RedTaq polymerase

Red colored Taq for easy viewing and loading
3U/ µl, Buffer contains MgCl2
PCR positive controls provided with every purchase
Red colored Taq for easy viewing and loading

SB/PCN 15

300 Units

RedTaq polymerase

Red colored Taq for easy viewing and loading
3U/ µl, MgCl2 provided separately
PCR positive controls provided with every purchase
Red colored Taq for easy viewing and loading

SB/PCN 16

300 Units

 RedTaq Master-Mix

Red Taq Polymerase -based kit
2X -mix (make up volume with template, primers and water)

SB/PCN 25 

5 x 1 ml

AMV Reverse Transcriptase

RNA directed DNA polymerase, synthesizes first strand cDNA 
Can reverse transcribe long RNAs (up to 10 kb)
Success of reaction heavily depends on RNA quality

SB/MFE 09

100 Units

M-MuLV Reverse Transcriptase

RNA directed DNA polymerase
Native enzyme, has RNaseH activity
Synthesizes first strand cDNA
Can reverse transcribe RNAs upto 3 kb long
Success of reaction heavily depends on RNA quality

SB/MFE 10

1000 Units

M-MuLV Reverse Transcriptase
(RNase H Negative)

RNA directed DNA polymerase
Genetically modified, RNase H negative
Synthesizes first strand cDNA
Can reverse transcribe RNAs up to 7 kb long
Success of reaction heavily depends on RNA quality

SB/MFE 10 A

1000 Units

RT-PCR Kit (M-MuLVbased)

For mRNA/ cDNA of ~3.0 kb
Robust amplification
5 control reactions

SB/PCN 28

10 Reactions

RT-PCR Kit (M-MuLV,
Single tube)

For mRNA/ cDNA of ~3.0 kb
RT and PCR are performed in same tube
Robust amplification
3 control reactions

SB/PCN 29

10 Reactions

RT-PCR Kit (AMV)

For mRNA / cDNA of >3.0 kb
Robust amplification
5 control reactions

SB/PCN 26

10 Reactions

RT-PCR Kit (AMV, Single tube)

For mRNA/ cDNA of >3.0 kb
RT and PCR in same tube
Robust amplification
3 control reactions

SB/PCN 27

10 Reactions

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