Screening for Transgenic plants

Genomic DNA isolated
Specific Primers developed
PCR based detection with internal PCR controls

SB/CSS 08 <10 plants

SB/CSS 09 <100 plants

SB/CSS 10 <1,000 plants

Transgene Copy No. detection

SYBR Green based protocol, performed  in triplicates
Copies of genes inserted within transgenic genome
Relative quantification based on house-keeping gene copy no.

SB/RTP 01

Point of Integration detected (Event Characterization)

Flanking regions cloned and sequenced
Sequence data provided
Site of integration identified
Validation of data

SB/CSS 07

Zygosity tests developed

For Parental seed purity testing
Zygosity test protocols for novel transgenic plants developed and validated
Based on flanking sequence data (Service CSS 07)

SB/CSS 11

Hybrid Seed Purity Tested

Ready protocol for Bt-cotton
A batch consists is of 100 individual  seeds
Percentage  of impurity (parental seeds, other non-Bt hybrid seeds, etc.) detected
Fast protocol, can test upto 100 independent  batches per day
Reproducible upto ±1% error

SB/CSS 12