Restriction Endonucleases Cutting Site Buffer Catalogue No Pack Size

Acc I

GT*MKAC

CB 4

SB/REN 001

500 units

Apa I

GGGCC*C

CB 4 + BSA

SB/REN 002A

500 units

Ava I

C*YCGRG

CB 4

SB/REN 003A

500 units

BamH I

G*GATCC

CB 3 + BSA

SB/REN 004A

2000 units

Bcl I

T*GATCA

CB 3

SB/REN 005A

500 units

Bgl I

GCCNNNN*NGGC

CB 3

SB/REN 006A

500 units

Bgl II

A*GATCT

CB 3

SB/REN 007A

500 units

Cla I

AT*CGAT

CB 4 + BSA

SB/REN 008A

500 units

EcoR I

G*AATTC

CB 1

SB/REN 009A

5000 units

EcoR V

GAT*ATC

CB 3 + BSA

SB/REN 010

500 units

Hind III

A*AGCTT

CB 2

SB/REN 011A

5000 units

Hinf I

G*ANTC

CB 4

SB/REN 012A

200 units

Hpa I

GTT*AAC

CB 4

SB/REN 013 A

500 units

Hpa II

C*CGG

CB 1

SB/REN 014A

500 units

Kpn I

GGTAC*C

CB 1

SB/REN 015A

1000 units

Mbo I

*GATC

CB 4

SB/REN 016

500 units

NdeI

CA*TATG

CB 1

SB/REN 017A

500 units

Nco I

C*CATGG

CB 3

SB/REN 018

500 units

Not I

GC*GGCCGC

CB 3 + BSA

SB/REN 019

500 units

Nru I

TCG*CGA

CB 3

SB/REN 020

500 units

Pst I

CTGCA*G

CB 3 + BSA

SB/REN 021A

2000 units

Pvu II

CAG*CTG

CB 2

SB/REN 022A

500 units

Sal I

G*TCGAC

CB 3 + BSA

SB/REN 023A

500 units

Sau96 I

G*GNCC

CB 4

SB/REN 024

500 units

Sfi I

GGCCNNNN*NGGCC

CB 4 + BSA

SB/REN 025

500 units

Sma I

CCC*GGG

CB 4

SB/REN 026A

500 units

Spe I

A*CTAGT

CB 4 + BSA

SB/REN 027 A

200 units

Ssp I

AAT*ATT

CB 1

SB/REN 028

200 units

Stu I

AGG*CCT

CB 4

SB/REN 029

200 units

Taq I

T*CGA

CB 4 + BSA

SB/REN 030A

500 units

Xba I

T*CTAGA

CB 4 + BSA

SB/REN 031

500 units

Xho I

C*TCGAG

CB 4 + BSA

SB/REN 032

500 units

Xma I

C*CCGGG

CB 4 + BSA

SB/REN 033A

200 units

Xmn I

GAANN*NNTTC

CB 4 + BSA

SB/REN 034A

500 units

          Single letter code: Y = C or T M = A or C K = G or T N = A or C or G or T R = A or G

          Modifying Enzymes 

Modifying Enzyme Specifications Catalogue No Pack Size

Terminal Transferase

Extends 3'-OH terminus
Template independent polymerase

SB/MFE 01

100 Units

Agarase

Cleaves agarose subunits
Agarose gel pieces can be digested and DNA fragments eluted

SB/MFE 02

50 Units

T4 Polynucleotide 
Kinase

Catalyzes transfer and exchange of inorganic phosphate at 3' end of DNA

SB/MFE 03

250 Units

Alkaline Phosphatase (Calf Intestine Phosphatase)

Catalyzes removal of 5' phosphate group
Obtained from Calf Intestinal mucosa

SB/MFE 04

200 Units

Uracil DNA Glycosylase

Catalyzes release of free Uracil

SB/MFE 05

200 Units

T4 DNA Ligase

Ligates blunt/ cohesive ends of DNA
Repairs single stranded nicks in duplex DNA/ RNA or hybrid
Tested for absence of Nucleases

SB/MFE 06

 

SB/MFE 06 S

1,00,000 Units

 

50,000 Units

DNA Polymerase I (klenow)

DNA polymerization  at 5'  to 3' direction
Has 3' to 5' exonuclease activit y

SB/MFE 07

100 Units

T7 RNA Polymerase

Used for in vitro synthesis of RNA from single stranded or double stranded DNA

SB/MFE 08

1000 Units

AMV Reverse 
Transcriptase

RNA directed DNA polymerase
Synthesizes first strand cDNA
Can reverse transcribe long RNAs (up to 10 kb)
Success of reaction heavily depends on RNA quality

SB/MFE 09

100 Units

M-MuLV Reverse Transcriptase

RNA directed DNA polymerase,
Native enzyme, has RNase H activity
Synthesizes first strand cDNA
Can reverse transcribe RNAs upto 3 kb long
Success of reaction heavily depends on RNA quality

SB/MFE 10

1000 Units

M-MuLV Reverse Transcriptase (RNase H Negative)

RNA directed DNA polymerase
Genetically modified, RNase H negative
Synthesizes first strand cDNA
Can reverse transcribe RNAs upto 7 kb long
Success of reaction heavily depends on RNA quality

SB/MFE 10A

1000 Units

DNAseI (RNase Free)

Endonuclease that cleaves DNA nonspecifically

SB/MFE 11

1000 Units

RNase H

Endoribonuclease
Degrades phosphodiester bonds of RNA- DNA hybrids during first strand cDNA synthesis

SB/MFE 12

100 Units

Ribonuclease A (DNase Free)

Powder; Molecular Biology Grade

SB/MFE 13
SB/MFE 13 S

500 mg
100 mg

Quick Ligase

Ligates blunt / cohesive ends of DNA
5 mins Ligation
Works the best with E. coli CB5α Competent cells 

SB/MFE 14

100 Ligations
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